![]() ![]() The octylamine ligands in the core of the bead are multimodal, being both hydrophobic and positively charged. These internalized ligands bind various contaminants strongly over a wide range of pH and salt concentrations. Using Capto Core 700 allows higher flow rates (1) and significantly higher sample loads than traditional GF (typically several column volumes compared with 0.1 to 0.3 column volumes in GF). The aim of this work was to evaluate Capto Core 700 chromatography medium as an alternative to current chromatography technologies used in vaccine processes. An experiment to determine the binding capacity and window of operation with Capto Core 700 for Madin- Darby canine kidney (MDCK) cell protein was performed using PreDictor 96-well plates for high-throughput process development. The approach taken for designing a purification process for flu vaccine is also described (Fig 1). Many infectious diseases are controlled using vaccines designed from subunits or whole viral structures, whereas other genetic diseases and cancers are being treated by viruses used as vehicles for delivering genetic material in gene therapy or as therapeutic agents in virotherapy protocols.įinally, the process economy impact of Capto Core 700 compared with Sepharose 4 Fast Flow, a GF medium often used in the vaccine manufacturing industry today, was also investigated.Viral vectors and viral vaccines are invaluable tools in prevention and treatment of diseases. Viral vectors and vaccines are produced in different platforms, from traditional embryonated chicken eggs to more advanced cell cultures. All these expression systems, like most cells and cellular tissues, are known to spontaneously release extracellular vesicles (EVs). EVs share similar sizes, biophysical characteristics and even biogenesis pathways with enveloped viruses, which are currently used as key ingredients in a number of viral vectors and licensed vaccine products. ![]()
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